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primescript tm one step rt pcr kit ver  (TaKaRa)


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    Structured Review

    TaKaRa primescript tm one step rt pcr kit ver
    Primescript Tm One Step Rt Pcr Kit Ver, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+tm+one+step+rt+pcr+kit+ver/PrimeScript+One+Step+RT-PCR+Kit+Ver%2E2/pmc12426205-87-6-13
    Average 96 stars, based on 667 article reviews
    primescript tm one step rt pcr kit ver - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Fat:

    Article Title: Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori.
    Article Snippet: .. Fat body of silkworms (3 rd day, fifth instar) was collected and homogenized for total RNA extraction by using Trizol Reagent (Takara, Dalian, China), and the first-strand cDNA was synthesized using PrimeScript TM One Step RT-PCR Kit Ver. ..

    RNA Extraction:

    Article Title: Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori.
    Article Snippet: .. Fat body of silkworms (3 rd day, fifth instar) was collected and homogenized for total RNA extraction by using Trizol Reagent (Takara, Dalian, China), and the first-strand cDNA was synthesized using PrimeScript TM One Step RT-PCR Kit Ver. ..

    Synthesized:

    Article Title: Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori.
    Article Snippet: .. Fat body of silkworms (3 rd day, fifth instar) was collected and homogenized for total RNA extraction by using Trizol Reagent (Takara, Dalian, China), and the first-strand cDNA was synthesized using PrimeScript TM One Step RT-PCR Kit Ver. ..

    One Step RT-PCR:

    Article Title: Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori.
    Article Snippet: .. Fat body of silkworms (3 rd day, fifth instar) was collected and homogenized for total RNA extraction by using Trizol Reagent (Takara, Dalian, China), and the first-strand cDNA was synthesized using PrimeScript TM One Step RT-PCR Kit Ver. ..

    Article Title: Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak
    Article Snippet: .. Qualified RNA from testicular tissues of three yaks and three cattleyaks was detected using PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara), and the amplification procedure was: reverse transcription at 50°C for 30 min, pre-denaturation at 94°C for 2 min, and 30 cycles of denaturation at 94°C for 30 s, annealing at 59°C for 30 s and extension at 72°C for 20 s, respectively. ..

    Article Title: Global Transcriptomic Analysis Reveals Insights into the Response of ‘Etrog’ Citron ( Citrus medica L.) to Citrus Exocortis Viroid Infection
    Article Snippet: Total RNA of approximately 0.1 g of citrus symptomatic leaves was extracted using EASYspin Plus Complex Plant RNA Kit (Aidlab Biotech, Beijing, China), according to the manufacturer’s instructions. .. Based on the sequences of CEVd (371 bp), one-step RT-PCR analysis using PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara, Beijing, China) was conducted to synthesize full-length cDNA of CEVd-dimer using CEVd-specific primers (CEVd-For: 5’-GGAAACCTGGAGGAAGTCGAG-3’ and CEVd-Rev: 5’-CCGGGGATCCCTGAAGGACTT-3’) [ ]. .. The dimeric products, which were amplified by one-step RT-PCR, were ligated overnight at 4°C using the pGEM-T Easy Vector System (Promega, Beijing, China) to obtain pGEM-CEVd-dimers, which were dimeric linker products.

    Article Title: Complete genome analysis and characterization of neurotropic dengue virus 2 cosmopolitan genotype isolated from the cerebrospinal fluid of encephalitis patients
    Article Snippet: To test ICFs for the presence of DENV, RNA was extracted from them by using viral RNA mini kit (Quiagen, Hilden, Germany) according to the manufacturer’s instruction. .. Then, PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara Bio Inc., Shiga, Japan) was used following the manufacturer’s instruction. ..

    Article Title: Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Hippocampus erectus
    Article Snippet: .. Since the genome structure of Hippocampus erectus astro-like virus 1 (abbreviated as HeAstV1) is distinct from other members of the family Astroviridae , RT-PCR of the original sample was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Beijing, China), with primers designed based on the obtained consensus sequence to further confirm the viral genome ( ). ..

    Article Title: Antiviral Cytokine Response in Neuroinvasive and Non-Neuroinvasive West Nile Virus Infection
    Article Snippet: .. Samples identified as positive using the real-time RT-PCR assay were subjected to conventional RT-PCR using pan-flavi primers targeting the NS5 gene (FP: 5′-TACAACATGATGGGVAARAGAGAGA-3′, RP: 5′-AGCATGTCTTCYGTBGTCATCCAYT-3′) to amplify 1,085 bp product [ ] by use of PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara Bio Inc, Kusatsu, Japan). ..

    Article Title: Molecular characterization of Coxsackievirus A2 isolated from hand, foot and mouth disease in eastern China
    Article Snippet: .. PCR amplification was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). ..

    Amplification:

    Article Title: Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak
    Article Snippet: .. Qualified RNA from testicular tissues of three yaks and three cattleyaks was detected using PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara), and the amplification procedure was: reverse transcription at 50°C for 30 min, pre-denaturation at 94°C for 2 min, and 30 cycles of denaturation at 94°C for 30 s, annealing at 59°C for 30 s and extension at 72°C for 20 s, respectively. ..

    Article Title: Molecular characterization of Coxsackievirus A2 isolated from hand, foot and mouth disease in eastern China
    Article Snippet: .. PCR amplification was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). ..

    Reverse Transcription:

    Article Title: Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak
    Article Snippet: .. Qualified RNA from testicular tissues of three yaks and three cattleyaks was detected using PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara), and the amplification procedure was: reverse transcription at 50°C for 30 min, pre-denaturation at 94°C for 2 min, and 30 cycles of denaturation at 94°C for 30 s, annealing at 59°C for 30 s and extension at 72°C for 20 s, respectively. ..

    Polymerase Chain Reaction:

    Article Title: Molecular characterization of Coxsackievirus A2 isolated from hand, foot and mouth disease in eastern China
    Article Snippet: Viral RNA was extracted from the 140 μl culture supernatants using the QIAamp Viral RNA Mini Kit (QIAGEN, California, USA) as per the instructions. .. The PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China), with the following primer pairs AOS (CCNTGGATHAGYAACCANCAYT) and AOAS (GGRTAANCCRTCRTARAACCAYTG), was used to perform reverse transcription PCR (RT-PCR) [ ] to obtain the partial VP1 region sequences. .. PCR-positive products were sequenced using an ABI 3130 Genetic Analyser (Applied Biosystems, Inc., USA) at Hangzhou Tsingke Biotechnology Co. (Hangzhou, Zhejiang Province, China) for sequencing.

    Article Title: Molecular characterization of Coxsackievirus A2 isolated from hand, foot and mouth disease in eastern China
    Article Snippet: .. PCR amplification was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular characterization of Coxsackievirus A2 isolated from hand, foot and mouth disease in eastern China
    Article Snippet: Viral RNA was extracted from the 140 μl culture supernatants using the QIAamp Viral RNA Mini Kit (QIAGEN, California, USA) as per the instructions. .. The PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China), with the following primer pairs AOS (CCNTGGATHAGYAACCANCAYT) and AOAS (GGRTAANCCRTCRTARAACCAYTG), was used to perform reverse transcription PCR (RT-PCR) [ ] to obtain the partial VP1 region sequences. .. PCR-positive products were sequenced using an ABI 3130 Genetic Analyser (Applied Biosystems, Inc., USA) at Hangzhou Tsingke Biotechnology Co. (Hangzhou, Zhejiang Province, China) for sequencing.

    Article Title: Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Hippocampus erectus
    Article Snippet: .. Since the genome structure of Hippocampus erectus astro-like virus 1 (abbreviated as HeAstV1) is distinct from other members of the family Astroviridae , RT-PCR of the original sample was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Beijing, China), with primers designed based on the obtained consensus sequence to further confirm the viral genome ( ). ..

    Article Title: Antiviral Cytokine Response in Neuroinvasive and Non-Neuroinvasive West Nile Virus Infection
    Article Snippet: .. Samples identified as positive using the real-time RT-PCR assay were subjected to conventional RT-PCR using pan-flavi primers targeting the NS5 gene (FP: 5′-TACAACATGATGGGVAARAGAGAGA-3′, RP: 5′-AGCATGTCTTCYGTBGTCATCCAYT-3′) to amplify 1,085 bp product [ ] by use of PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara Bio Inc, Kusatsu, Japan). ..

    Virus:

    Article Title: Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Hippocampus erectus
    Article Snippet: .. Since the genome structure of Hippocampus erectus astro-like virus 1 (abbreviated as HeAstV1) is distinct from other members of the family Astroviridae , RT-PCR of the original sample was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Beijing, China), with primers designed based on the obtained consensus sequence to further confirm the viral genome ( ). ..

    Sequencing:

    Article Title: Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Hippocampus erectus
    Article Snippet: .. Since the genome structure of Hippocampus erectus astro-like virus 1 (abbreviated as HeAstV1) is distinct from other members of the family Astroviridae , RT-PCR of the original sample was performed using the PrimeScript TM One Step RT-PCR Kit Ver.2 (TaKaRa, Beijing, China), with primers designed based on the obtained consensus sequence to further confirm the viral genome ( ). ..

    Quantitative RT-PCR:

    Article Title: Antiviral Cytokine Response in Neuroinvasive and Non-Neuroinvasive West Nile Virus Infection
    Article Snippet: .. Samples identified as positive using the real-time RT-PCR assay were subjected to conventional RT-PCR using pan-flavi primers targeting the NS5 gene (FP: 5′-TACAACATGATGGGVAARAGAGAGA-3′, RP: 5′-AGCATGTCTTCYGTBGTCATCCAYT-3′) to amplify 1,085 bp product [ ] by use of PrimeScript TM One Step RT-PCR Kit Ver.2 (Takara Bio Inc, Kusatsu, Japan). ..



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